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| Status |
Public on May 04, 2015 |
| Title |
Microarray analysis of mouse gene expression in metronomic cyclophosphamide-treated human glioma U251 xenografts implanted in scid mice |
| Platform organism |
Mus musculus |
| Sample organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cyclophosphamide (CPA) treatment on a six-day repeating metronomic schedule induces a dramatic, innate immune cell-dependent regression of implanted gliomas. However, little is known about the underlying mechanisms of innate immune cell mobilization and recruitment, or about the role of DNA damage and cell stress response pathways in eliciting the anti-tumor immune responses linked to tumor regression. To address these questions, we compared untreated and 6-day metronomic cyclophosphamide-treated human U251 glioblastoma xenografts by mouse microarray analysis to identify responsive mouse (host) cell-specific factors.
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| Overall design |
Human glioma U251 tumors were implanted sc in scid immunodeficient mice then treated with cyclophosphamide at 140 mg/kg every 6 days. Tumors were collected 6 days after the second cyclophosphamide treatment and also 6 days after the third cyclophosphamide treatment. Tumor RNA was then analyzed on two color Agilent mouse expression microarrays comparing cyclophosphamide-treated RNA to untreated control tumor RNA.
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| Contributor(s) |
Doloff JC, Waxman DJ |
| Citation(s) |
25952672 |
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| Submission date |
Aug 27, 2014 |
| Last update date |
Nov 01, 2017 |
| Contact name |
David J. Waxman |
| E-mail(s) |
[email protected]
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| Organization name |
Boston University
|
| Department |
Department of Biology and Bioinformatics Program
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| Street address |
5 Cummington Mall
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
| GSM1491702 |
U251 tumor_2nd metroCPA (pool JD9 and JD10) versus U251 tumor_control (pool JD5 and JD6)_Mouse microarray |
| GSM1491703 |
U251 tumor_3rd metroCPA (pool JD7 and JD8) versus U251 tumor_control (pool JD5 and JD6)_Mouse microarray |
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| This SubSeries is part of SuperSeries: |
| GSE60913 |
Gene expression in metronomic cyclophosphamide-treated glioma xenografts |
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| Relations |
| BioProject |
PRJNA259777 |
