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Series GSE60471 Query DataSets for GSE60471
Status Public on Feb 18, 2016
Title Drosophila melanogaster high resolution developmental transcriptomic time-course
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three phyla has led us to ask if all animals pass through a universal definitive stage as a consequence of ancestral constraints on animal development. Previous work has suggested that HOX genes may comprise such a ‘zootypic’ stage, however this hypothetical stage has hitherto resisted systematic analysis. We have examined the embryonic development of ten different animals each of a fundamentally different phylum, including a segmented worm, a flatworm, a roundworm, a water bear, a fruitfly, a sea urchin, a zebrafish, a sea anemone, a sponge, and a comb jelly. For each species, we collected the embryonic transcriptomes at ~100 different developmental stages and analyzed their gene expression profiles. We found dynamic gene expression across all of the species that is structured in a stage like manner. Strikingly, we found that animal embryology contains two dominant modules of zygotic expression in terms of their protein domain composition: one involving proliferation, and a second involving differentiation. The switch between these two modules involves induction of the zootype; which in addition to homeobox containing genes, also involves Wnt and Notch signaling as well as forkhead domain transcription factors. Our results provide a systematic characterization of animal universality and identify the points of embryological constraints and flexibility.
 
Overall design 91 single embryo samples.
 
Contributor(s) Levin M, Anavy L, Yanai I
Citation(s) 26886793
Submission date Aug 18, 2014
Last update date May 15, 2019
Contact name Itai Yanai
E-mail(s) [email protected]
Organization name Technion - Israel Institute of Technology
Department Biology
Lab Yanai
Street address Technion City
City Haifa
ZIP/Postal code 30200
Country Israel
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (91)
GSM1480615 Metazome_Drosophila_timecourse_sample_0001
GSM1480616 Metazome_Drosophila_timecourse_sample_0002
GSM1480617 Metazome_Drosophila_timecourse_sample_0003
This SubSeries is part of SuperSeries:
GSE70185 The mid-developmental transition and the evolution of animal body plans
Relations
BioProject PRJNA258368
SRA SRP045563

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60471_CEL-Seq_barcode_V2.tab.gz 325 b (ftp)(http) TAB
GSE60471_DM_GEO_sample_sheet.tab.gz 1.0 Kb (ftp)(http) TAB
GSE60471_DM_exp.tab.gz 1.0 Mb (ftp)(http) TAB
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Raw data are available in SRA
Processed data are available on Series record
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