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| Status |
Public on Sep 11, 2014 |
| Title |
Extensive translation of small Open Reading Frames revealed by Poly-Ribo-Seq |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We present a genome-wide assessment of small open reading frames (smORF) translation by ribosomal profiling of polysomal fractions in Drosophila S2 cell. In this way, mRNAs bound by multiple ribosomes and hence actively translated can be isolated and distinguished from mRNAs bound by sporadic, putatively non-productive single ribosomes or ribosomal subunits.
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| |
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| Overall design |
Ribosomal profiling of large and small polysomal fractions in Drosophila S2 cells to assess translation of smORFs
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| |
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| Contributor(s) |
Aspden JL, Eyre-Walker YC, Couso JP |
| Citation(s) |
25144939 |
| |
| Submission date |
Aug 13, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Juan Pablo Couso |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Sussex
|
| Street address |
Falmer
|
| City |
Brighton |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA258156 |
| SRA |
SRP045475 |
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