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| Status |
Public on Sep 29, 2014 |
| Title |
Human stem cell and mouse transchromosomic (hsa11) embryonic stem cell (with and without ectopic ZNF91 expression) ChIP-seq |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We compared the binding patterns in embryonic stem cells of KAP1 and KRAB Zinc Finger (KZNF) proteins as well as H3K4me3 DNA under several conditions. Native human stem cells. Mouse stem cells containing a transchromosomic copy of human chromosome 11, with and without the introduction of plasmids containing KRAB Zinc Finger sequences. We show that certain KZNFs are responsible for the repression of certain retrotransposons in embryonic stem cells, preventing their spread across the genome.
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| Overall design |
ChIP-seq of HESCs and mouse TC11 ESCs with ZNF91 plasmids and with empty vector plasmids. ChIP of KAP1, ZNF486, H3K4me3. At least 2 replicates of each condition.
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| Contributor(s) |
Jacobs FM, Greenberg D, Nguyen N, Haeussler M, Ewing AD, Katzman S, Paten B, Salama SR, Haussler D |
| Citation(s) |
25274305 |
| |
| Submission date |
Aug 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Sofie Salama |
| E-mail(s) |
[email protected]
|
| Phone |
8314592814
|
| Organization name |
Univerity of California, Santa Cruz
|
| Department |
Center for Biomolecular Science and Engineering
|
| Lab |
Haussler
|
| Street address |
MS: CBSE, 1156 High St
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95060 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE60211 |
An evolutionary arms race between KRAB zinc finger genes 91/93 and SVA/L1 retrotransposons |
|
| Relations |
| BioProject |
PRJNA257759 |
| SRA |
SRP045340 |
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