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| Status |
Public on May 23, 2007 |
| Title |
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To characterize the differentiation by Sox2 KO, we performed microarray analyses of mouse ES cell line 2TS22C during the time-course being induced of Sox2 KO
Keywords: development or differentiation design,time series design
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| Overall design |
Inducible Sox2 KO cell line 2TS22C were assigned to eight experimental groups and cultured in GMEM medium (SIGMA) with LIF at 37°C in an atmosphere of 5% CO2 until the specified time point before the harvest: (1)for controls, cultured in the absence of Tc; (2) for 3h after the administration of Tc; (3) for 6h; (4) for 12h; (5) for 24h; (6) for 48h; (7) for 72h; (8) for 96h.
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| Contributor(s) |
Masui S, Shimosato D, Toyooka Y, Nakatake Y, Yagi R, Takahashi K, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H |
| Citation(s) |
17515932 |
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| Submission date |
Sep 21, 2006 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
[email protected]
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| Phone |
410-558-8359
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| Organization name |
NIH
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| Department |
National Institute on Aging
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| Lab |
Lab of Genetics
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| Street address |
251 Bayview Blvd, Suite 100, 10C
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL4358 |
NIA Mouse 44K Microarray v2.2 (Whole Genome 60-mer Oligo) |
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| Samples (12)
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| GSM137091 |
Sox2-KO, Tet(-), 0hr - #1(251411710001) |
| GSM137092 |
Sox2-KO, Tet(-), 0hr - #2(251411710002) |
| GSM137093 |
Sox2-KO, Tet(+), 3hr - #1(251411710003) |
| GSM137094 |
Sox2-KO, Tet(+), 6hr - #1(251411710005) |
| GSM137095 |
Sox2-KO, Tet(+), 6hr - #2(251411710006) |
| GSM137096 |
Sox2-KO, Tet(+), 12hr - #1(251411710007) |
| GSM137097 |
Sox2-KO, Tet(+), 24hr - #1(251411710008) |
| GSM137098 |
Sox2-KO, Tet(+), 24hr - #2(251411710009) |
| GSM137099 |
Sox2-KO, Tet(+), 48hr - #1(251411710010) |
| GSM137100 |
Sox2-KO, Tet(+), 72hr - #1(251411710011) |
| GSM137101 |
Sox2-KO, Tet(+), 72hr - #2(251411710012) |
| GSM137102 |
Sox2-KO, Tet(+), 96hr - #1(251411710013) |
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| Relations |
| BioProject |
PRJNA97323 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5895_RAW.tar |
399.2 Mb |
(http)(custom) |
TAR (of TIFF, TXT) |
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