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Series GSE57776 Query DataSets for GSE57776
Status Public on Jul 31, 2015
Title Transcriptome of Smooth Muscle Cells, Interstitial Cells of Cajal and PDGFRα+ Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Genome scale expression data on absolute numbers of gene isoforms offer essential clues for cellular functions and biological processes. Gastrointestinal (GI) motility is regulated by smooth muscle cells (SMC) closely contacted with interstitial cells of Cajal (ICC) and fibroblast-like PDGFRα+ cells (PαC), forming an electrical syncytium. To uncover genetic identifies and cellular functions of the cells, we isolated these three cell populations from mouse small intestine and colon, obtained the transcriptome for each type of cells, and built each cell type transcriptome browser. To our knowledge, this is the first genetic resource providing a comprehensive reference for all mRNA transcripts expressed in these unique GI cell populations. Integration of these data with the UCSC genome browser revealed novel cell-specific markers, ion channel and transporter isoforms, and unique cellular and biological functions of these cells in GI physiology. Our transcriptome browsers bring new insight into the alternative expression of genes in different types of the cells and provides references for future functional studies.
 
Overall design mRNA profiles of SMC, ICC and PDGFRa cells isolated from mouse jejunum and colon were generated by deep sequencing using Illumina Hiseq2000..
 
Contributor(s) Park C, Townsend J, Redelman D, Shen T, Sanders KM, Ro S
Citation(s) 26241044
Submission date May 19, 2014
Last update date May 15, 2019
Contact name Seungil Ro
E-mail(s) [email protected]
Phone 775-784-1462
Organization name University of Nevada School of Medicine
Department Physiology and Cell Biology
Street address 1664 North Virginia St
City Reno
State/province NV
ZIP/Postal code 89557
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (10)
GSM1388406 SMC Jejunum
GSM1388407 SMC Colon
GSM1388408 ICC Jejunum
Relations
BioProject PRJNA248074
SRA SRP042078

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Supplementary file Size Download File type/resource
GSE57776_isoforms_fpkm_tracking.xlsx.gz 17.9 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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