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Series GSE5751

Query DataSets for GSE5751

Status

Public on Jan 08, 2007

Title

The early post-germinative embryo and endosperm transcriptomes in Arabidopsis

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Arabidopsis seed germination is coordinated with the strong induction of metabolic pathways required for the mobilisation and utilization of seed storage reserves. These are essential to support the seedling before the establishment of photoauxotrophic growth. The activity of genes encoding enzymes required for lipid mobilisation is regulated largely at the level of transcription, but our knowledge of how this regulation occurs is extremely limited. After germination the rate of lipid reserve mobilisation is determined by the carbohydrate status of the seedling and by the osmotic potential of the growth substrate. The plant response to both of these requires the action of the hormone abscisic acid (ABA).
We have shown that this regulation is tissue specific (Penfield et al., 2004 Plant Cell 16, 2705-2718), and that although lipid breakdown in the embryo is inhibited by ABA, lipid breakdown in the endosperm tissues is not. Furthermore, in many species the action of the endosperm is central to the processes controlling seed germination, yet very little is known about gene expression in this tissue, or of the function of the endosperm in mature Arabidopsis seeds. Mature Arabidopsis seeds can be dissected into embryo and endosperm/seed coat fractions, with the latter fraction containing RNA only from the endosperm as the seed coat cells undergo programmed cell death during the latter stages of seed development. In this experiment we divide seeds into embryo and endosperm tissues and transcript profile both shortly after germination, or when germination and lipid reserve mobilisation are inhibited by ABA or by the gibberellin biosynthesis inhibitor paclobutrazol. In this way we can discover the endosperm transcriptome and uncover candidate regulators of lipid mobilisation by searching for genes showing differential expression between embryo and endosperm before and after ABA treatment.

Experimenter name = Steven Penfield
Experimenter phone = 01904 328759
Experimenter fax = 01904 328762
Experimenter department = Department of Biology
Experimenter institute = Centre for Novel Agricultural Products
Experimenter address = University of York
Experimenter address = PO BOX 373
Experimenter address = York
Experimenter zip/postal_code = YO10 5YW
Experimenter country = UK
Keywords: organism_part_comparison_design

Overall design

18 samples were used in this experiment

Contributor(s)

Penfield S, Graham I, Townsend H, Emmerson Z, Schildknecht B

Citation missing

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Submission date

Sep 01, 2006

Last update date

Aug 28, 2018

Contact name

Nottingham Arabidopsis Stock Centre (NASC)