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| Status |
Public on Jan 08, 2007 |
| Title |
In vitro tracheary element transdifferentiation of Col-0 suspension cells. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment was annotated by TAIR (http://arabidopsis.org).
Col-0 suspension cells provided by Dr. C. Koncz and Dr. M. Umeda. 15 ml of Arabidopsis Col-0 suspension cells (Mathur et al. 1998) was transferred to 35 ml of a fresh modified Murashige and Skoog (MS) medium supplemented with 1 ug /ml 2,4-dichlorphenoxyacetic acid and 3% sucrose every 7 day, and cultured on a rotary shaker at 120 rpm in the dark at 22 C for subculture. For xylem vessel element induction, a 7.5 ml aliquot of 7-day-old subcultured cells was transferred into 42.5 ml of fresh medium that included 1 uM brassinolide and 10 mM H3BO3, and cultured as described above. The frequency of xylem vessel element formation was calculated as the proportion of xylem vessel elements to the number of living cells and the vessel elements.
Experimenter name = Hiroo Fukuda
Experimenter phone = +81-3-5841-4463
Experimenter fax = +81-3-5841-4462
Experimenter department = H Fukuda Laboratory
Experimenter institute = University of Tokyo
Experimenter address = Department of Biological Science
Experimenter address = 2nd Buil.
Experimenter address = Hongo 7-3-1, Bunkyo-ku
Experimenter zip/postal_code = Tokyo 113-0033
Experimenter country = Tokyo
Keywords: compound_treatment_design; time_series_design;
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| |
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| Overall design |
12 samples were used in this experiment
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| |
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| Contributor(s) |
Fukuda H, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 01, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA97069 |
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