|
| Status |
Public on Jan 08, 2007 |
| Title |
The effects of mutants in stress response pathways on gene expression during senescence |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Many signalling pathways are involved in controlling gene expression during plant senescence. Pathways involving SA, JA and ethylene have a role in senescence but none are essential for the senescence process to occur. The aim of this experiment is to classify senescence-enhanced genes into groups depending on the signalling pathways that regulate them. This will provide useful information on the relative importance of each signalling pathway during senescence and allow us to separate potential senescence-specific genes and pathways from the stress response pathways.Mutants in genes in the ethylene pathway (ein2) and the jasmonate pathway (coi1) and the NahG transgenic plant which is defective in the salicylic acid pathway will be grown until the mid flowering stage. Fully developed green and partially senescent leaves will be harvested from the plants at this stage. In addition, two different lines of Arabidopsis (Col5 glabrous and Col-0) will be grown as controls. Leaves will be harvested from the two control plants before flowering (green) and at mid flowering as above. The control plants will be harvested at two stages to identify the senescence- enhanced genes. The effects of each mutation on the senescence related expression of these genes will then be studied.Mutant RNAs will be isolated in duplicate. The two control accessions will act as replicates for the wild type. Two wild type accessions will be used to reduce possible differences that could be observed the mutants due to slight differences in background.
Experimenter name = Vicky Buchanan-Wollaston
Experimenter phone = 01789 470382
Experimenter fax = 01789 470552
Experimenter address = Horticulture Research International
Experimenter address = Wellesbourne
Experimenter address = Warwick
Experimenter zip/postal_code = CV35 9EF
Experimenter country = UK
Keywords: genetic_modification_design; development_or_differentiation_design
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| |
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| Overall design |
10 samples were used in this experiment
|
| |
|
| Contributor(s) |
Buchanan-Wollaston V, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 01, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (10)
|
| GSM133729 |
Buchanan-Wollaston_A-1-bwoll-C0G_SLD |
| GSM133730 |
Buchanan-Wollaston_A-2-bwoll-C5G_SLD |
| GSM133731 |
Buchanan-Wollaston_A-3-bwoll-C0S_SLD |
| GSM133732 |
Buchanan-Wollaston_A-4-bwoll-C5S_SLD |
| GSM133733 |
Buchanan-Wollaston_A-5-bwoll-NG1_SLD |
| GSM133734 |
Buchanan-Wollaston_A-6-bwoll-NG2_SLD |
| GSM133735 |
Buchanan-Wollaston_A-7-bwoll-Ei1_SLD |
| GSM133736 |
Buchanan-Wollaston_A-8-bwoll-Ei2_SLD |
| GSM133737 |
Buchanan-Wollaston_A-9-bwoll-Co1_SLD |
| GSM133738 |
Buchanan-Wollaston_A-10-bwoll-Co2_SLD |
|
| Relations |
| BioProject |
PRJNA97029 |
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