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| Status |
Public on Jan 08, 2007 |
| Title |
Cold induced changes: differences between the Arabidopsis thaliana wild type and freezing sensitive mutants. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Arabidopsis sfr mutants are deficient in cold acclimation during exposure to coolnon-freezing temperatures. Although not visibly affected by the cold they have lost the ability to survive subsequent freezing. We plan to investigate how the sfr2 and sfr6 mutants respond to low temperature on the gene expression level. Wild type plants that have undergone identical treatments in parallel are necessary controls. The cold treatment of plants in the rosette stage (soil grown in a 8/16 hours day/night cycle) will be carried out in a cooled growth chamber at 4 degrees for 24 hours (same light regimetreatment starting/ending at the 4th hour of light). The aerial parts of the treated and untreated plants will be collected and frozen immediately in liquid nitrogen for RNA extraction. Comparison of the cold response of thousands of Arabidopsis genes in the wild type to the situation in our freezing sensitive mutants will enhance our understanding of the cold response itself and illuminate the effect of the mutations on the cold acclimation process.
Experimenter name = Irene Bramke
Experimenter phone = 01784 44 3770
Experimenter fax = 01784 43 4326
Experimenter address = Royal Holloway
Experimenter address = University of London
Experimenter address = School of Biological Sciences
Experimenter address = Bourne Building
Experimenter address = Laboratory 406
Experimenter zip/postal_code = TW20 OEX
Experimenter country = UK
Keywords: growth_condition_design; genetic_modification_design
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| Overall design |
6 samples were used in this experiment
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| |
|
| Contributor(s) |
Bramke I, Warren G, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
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| |
| Submission date |
Aug 31, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA96999 |
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