|
| Status |
Public on Jan 08, 2007 |
| Title |
Peroxisomal mdh mutant |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim is to study the function of peroxisomal NAD-malate dehydrogenase in fatty acid beta-oxidation, glyoxylate cycle and photorespiration. Both peroxisomal MDH genes (At2g22780 and At5g09660) have been knocked out with T-DNA insertions and a double mutant made. Double mutant seedlings are blocked in beta-oxidation - they are 2,4DB resistant and beta-oxidation genes are repressed. They carry out glyoxylate cycle as normal. Plants grow well in soil and produce seed. Microarray analysis will tell us the extent of changes in gene expression in the mutant. For microarray analysis seeds are stratified at 4 C on agar medium with 1/2 strength M&S salts and 1% sucrose for 2 days, then seedlings grown for 2 days at 20 C in the light (100 umol/m2/s). Triplicate samples will be grown for mutant and wild type (col-0) and RNA isolated from each.
Experimenter name = Itsara Pracharoenwattana
Experimenter phone = 0131 650 5316
Experimenter fax = 0131 650 5392
Experimenter department = Institute of Molecular Plant Sciences
Experimenter institute = University of Edinburgh
Experimenter address = Daniel Rutherford Building University of Edinburgh
Experimenter address = The King's Buildings
Experimenter address = Edinburgh
Experimenter zip/postal_code = EH9 3JH
Experimenter country = UK
Keywords: genetic_modification_design
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| |
|
| Overall design |
6 samples were used in this experiment
|
| |
|
| Contributor(s) |
Pracharoenwattana I, Smith S, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 25, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (6)
|
| GSM131844 |
Pracharoenwattana_1-1_mdhA_Rep1_ATH1 |
| GSM131845 |
Pracharoenwattana_1-2_mdhB_Rep2_ATH1 |
| GSM131846 |
Pracharoenwattana_1-3_mdhC_Rep3_ATH1 |
| GSM131847 |
Pracharoenwattana_1-4_ColA_Rep1_ATH1 |
| GSM131848 |
Pracharoenwattana_1-5_ColB_Rep2_ATH1 |
| GSM131849 |
Pracharoenwattana_1-6_ColC_Rep3_ATH1 |
|
| Relations |
| BioProject |
PRJNA96839 |
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