|
| Status |
Public on Aug 15, 2006 |
| Title |
Targets of AtWRKY27 in Arabidopsis leaves |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants.
Experimenter name = Shahid Mukhtar
Experimenter phone = +49-221-5062-310
Experimenter fax = +49-221-5062-353
Experimenter address = Max.Planck-Institute for Plant Breeding
Experimenter address = Dept. Plant Microbe Interactions
Experimenter address = Carl-von-Linne Weg 10
Experimenter address = Koeln
Experimenter zip/postal_code = 50829
Experimenter country = Germany
Keywords: genetic_modification_design
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| |
|
| Overall design |
4 samples were used in this experiment
|
| |
|
| Contributor(s) |
Mukhtar S, Somssich I, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 15, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA96023 |
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