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| Status |
Public on Aug 15, 2006 |
| Title |
Hydrogen peroxide stress and Zat12 over-expression in Arabidopsis. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
In this experiment we tested the transcriptome of transgenic Arabidopsis seedlings (5-day-old) constitutively expressing the zinc-finger protein Zat12 (At5g59820) under the control of the 35S-CaMV promoter (Zat12). The transcriptome of these seedlings was compared to that of wild type seedlings grown under the same conditions (WT) and to that of wild type seedlings grown under the same conditions and subjected to a hydrogen peroxide stress (WT+H2O2). Hydrogen peroxide treatment was performed by applying 20 mM hydrogen peroxide for 1 hour. In parallel to these experiments transgenic plants expressing Zat12 were subjected to a similar hydrogen peroxide stress (Zat12+H2O2). All treatments were performed with similar size and age seedlings grown in liquid culture (MS) and sampled at the same time as described by Davletova et al., 2005.
Experimenter name = Ron Mittler
Experimenter phone = 1-775-784-1384
Experimenter fax = 1-775-784-1650
Experimenter department = Dept. of Biochemistry
Experimenter institute = University of Nevada
Experimenter address = MS200
Experimenter address = Reno
Experimenter address = Nevada
Experimenter zip/postal_code = 89557
Experimenter country = USA
Keywords: genetic_modification_design; stimulus_or_stress_design
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| Overall design |
12 samples were used in this experiment
|
| |
|
| Contributor(s) |
Mittler R, Mittler R, Townsend H, Emmerson Z, Schildknecht B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 15, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (12)
|
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA96011 |
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