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Series GSE54750 Query DataSets for GSE54750
Status Public on Feb 06, 2014
Title RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides
Organism Cereibacter sphaeroides 2.4.1
Experiment type Expression profiling by high throughput sequencing
Summary A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1∆rnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3´-to-5´ exoribonucleases.
 
Overall design Total RNA from exponentially grown cultures (OD660 0,4) of 2.4.1 wild type and 2.4.1∆rnj has been isolated in triplicates using the hot phenol method and prepared for sequencing on a Illumina Genome Analyzer IIx machine.
 
Contributor(s) Rische-Grahl T, Weber L, Remes B, Förstner KU, Klug G
Citation(s) 24922065
Submission date Feb 06, 2014
Last update date May 15, 2019
Contact name Konrad U. Förstner
E-mail(s) [email protected]
Organization name ZB MED - Information Centre for Life Sciences
Department Information Services
Lab Förstner Lab
Street address Gleueler Str. 60
City Cologne
State/province North Rhine-Westphalia
ZIP/Postal code 50931
Country Germany
 
Platforms (1)
GPL18261 Illumina Genome Analyzer IIx (Rhodobacter sphaeroides 2.4.1)
Samples (2)
GSM1323506 Rhodobacter sphaeroides wild type
GSM1323507 Rhodobacter sphaeroides RNase J deletion mutant
Relations
BioProject PRJNA237547
SRA SRP036835

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54750_RAW.tar 25.4 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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