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| Status |
Public on Jan 13, 2021 |
| Title |
In vivo organization of bacterial transcription initiation complexes at a genome-scale [RNA-seq] |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA polymerase (RNAP) is essential for the transcription of genetic information encoded in genomes in all three domains of life. To determine the precise organization of bacterial transcription initiation complexes (TIC) in vivo, we exploited high-throughput sequencing to the DNA obtained from exonuclease-treated immunoprecipitates of the TIC. It reveals that sigma (σ) factor is mostly engaged in RNAP holoenzyme up to 13-14 nucleotides from transcription start site during abortive initiation.
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| Overall design |
Directional RNA-seq was generated in duplicates by using Illumina MiSeq
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| Contributor(s) |
Cho S, Cho Y, Lee E, Woo YI, Roe J, Kim SC, Cho B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 26, 2014 |
| Last update date |
Jan 13, 2021 |
| Contact name |
Byung-Kwan Cho |
| Organization name |
KAIST
|
| Lab |
Systems and Synthetic Biology Lab
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| Street address |
291 Daehak-ro
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| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
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| Platforms (1) |
| GPL17439 |
Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE54407 |
In vivo organization of bacterial transcription initiation complexes at a genome-scale |
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| Relations |
| BioProject |
PRJNA236562 |
| SRA |
SRP035860 |
