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| Status |
Public on Dec 01, 2014 |
| Title |
DNA methylation analysis of human atherosclerotic plaques in comparison to healthy mammary arteries |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We report differential DNA methylation results from human femoral atherosclerotic plaques compared to healthy mammary arteries. 5-methylcytosine containing DNA fragments are captured by methylcytosine binding domain - based MethylCapture assay. Captured fragments are sequenced using Illumina paired-end approach and mapped to human genome (hg19).
Results. Hypomethylation of chromosomal DNA predominates in atherosclerotic plaques. Chr14q32.2 was identified for the first time as an extensively hypomethylated area in atherosclerosis with highly induced expression of miR127, -136, -410, -431, and -432
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| Overall design |
DNA methylation in 8 human atherosclerotic plaques was compared to 5 healthy mammary arteries.
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| Contributor(s) |
Aavik E, Leppänen O, Bräsen J, Beschorner U, Zeller T, Braspenning M, van Criekinge W, Mäkinen K, Ylä-Herttuala S |
| Citation(s) |
25411193 |
| |
| Submission date |
Dec 22, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Einari Aavik |
| Organization name |
University of Eastern Finland
|
| Department |
A.I. Virtanen Institute
|
| Street address |
Neulaniementie 2
|
| City |
Kuopio |
| ZIP/Postal code |
FIN-70211 |
| Country |
Finland |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA232429 |
| SRA |
SRP034676 |
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