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| Status |
Public on Sep 24, 2013 |
| Title |
Expression data from WT and Eed KO neonatal mouse LT-HSC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Recent studies point to a pivotal role of polycomb repressive complex 2 (PRC2) in stem cell function and cancer. Loss of function approaches targeting individual PRC2 subunits have however generated findings that are difficult to reconcile.
Here, we prevent assembly of both Ezh1- and Ezh2-containing PRC2 complexes by conditional deletion of Eed, a core subunit, and assess glodbal gene expression changs in LT-HSCs.
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| Overall design |
LT-HSC (long-term hematopoietic stem cells) were sorted from bone marrow of 9-day old wild type (Lin-Sca1+Kit+CD48-CD150+) or Eed KO animals (Lin-Sca1+CD48-CD150+). Each sample was double or triple sorted to ensure high purify.
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| Contributor(s) |
Xie H, Orkin S |
| Citation(s) |
24239285, 27630126 |
| |
| Submission date |
Sep 23, 2013 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Huafeng Xie |
| E-mail(s) |
[email protected]
|
| Phone |
6176326492
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA221146 |
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