|
| Status |
Public on Dec 01, 2013 |
| Title |
Expression data from fission yeast deletion mutant of Klf1, a C2H2 zinc finger-transcription factor, in G0 and VE phases |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
|
| Summary |
Fission yeast Schizosaccharomyces pombe is a model for studying cellular quiescence. Shifting to medium without a nitrogen-source induces proliferative cells to enter long-term G0 quiescence. Klf1 is a Kruppel-like transcription factor with a 7-amino acid-spaced C2H2-type zinc finger motif. The deletion mutant ∆klf1 normally divides in vegetative medium, but proliferation is not restored after long-term G0 quiescence. Cell biologic, transcriptomic, and metabolomic analyses revealed a unique phenotype of the ∆klf1 mutant in quiescence. Mutant cells had diminished transcripts related to signaling molecules for switching to differentiation. In contrast, proliferative metabolites for cell-wall assembly and antioxidants significantly increased. Further, the size of the ∆klf1 cells is markedly increased during quiescence due to the aberrant accumulation of calcofluor-positive chitin-like materials beneath the cell wall. After 4 weeks quiescence, the ability for reversible proliferation is lost, but energy metabolism is maintained. Klf1 thus plays a role in G0 phase longevity through enhancing the differentiation signal and suppressing metabolism for growth. If Klf1 is lost, S. pombe fails to maintain a constant cell size during quiescence.
|
| |
|
| Overall design |
Gene expression profile of fission yeast wild type cells and klf1-gene disruptant cells in proliferating state and in quiescent state.
Type of experiment: Comparing between wild type cells and klf1-gene disruptant cells in proliferating condition and in quiescent condition.
Experimental factor: Exponentially proliferating state in synthetic medium, EMM2, and quiescent G0 state in nitrogen-depleted synthetic medium, EMM2-N.
Quality control steps taken: All experiments were repeated twice in each condition.
|
| |
|
| Contributor(s) |
Shimanuki M, Uehara L, Pluskal T, Yoshida T, Kokubu A, Kawasaki Y, Yanagida M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 18, 2013 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Mizuki Shimanuki |
| E-mail(s) |
[email protected]
|
| Organization name |
Okinawa Institute of Science and Technology Graduate University
|
| Department |
Research Support Division
|
| Lab |
Biology Resource Section
|
| Street address |
1919-1, Tancha
|
| City |
Onna-son |
| State/province |
Okinawa |
| ZIP/Postal code |
904-0495 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA219487 |
Less...
More...