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| Status |
Public on Mar 03, 2015 |
| Title |
Transcriptomic Analysis of Non-small Cell Lung Cancer Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study was designed to understand the transcriptomic composition and the biological functions of cancer stem cells isolated from non-small cell lung cancer line (NSCLC)
Putative lung cancer stem cells were isolated from cancer cell lines based on expression of known stem cell surface markers: CD166, CD44 and EpCAM using the Fluorescence Activated Cell Sorter (FACS).
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| Overall design |
Affymetrix microarray were performed on cancer stem cells isolated from normal lung epithelial cells and lung cancer cell lines (A549 and NCI-H2170) using GeneChip Human Gene 1.0 ST array. The normal putative stem cells isolated from normal primary human bronchial/trachial epithelial cell line (PHBEC) was serve as control. Putative cancer stem cells isolated from A549 and NCI-H2170 cell lines are the treatment group. Each sample was performed in triplicate and total number of samples are five (n=5)
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| Contributor(s) |
Yahaya BH, Zakaria N |
| Citation(s) |
25881239 |
| |
| Submission date |
Sep 05, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Badrul Hisham Yahaya |
| E-mail(s) |
[email protected]
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| Phone |
+6045622539
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| Organization name |
Advanced Medical and Dental Institute
|
| Department |
Advanced Medical & Dental Institute
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| Lab |
Regenerative Medicine
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| Street address |
Bertam, Kepala Batas
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| City |
Kepala Batas |
| State/province |
Penang |
| ZIP/Postal code |
13200 |
| Country |
Malaysia |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA218111 |
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