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| Status |
Public on Jul 03, 2013 |
| Title |
Expression data from well-differentiated human bronchial epithelial cells infected with H1N1 Influenza isolates |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarrays to compare the gene expression profiles of different H1N1 isolates (seasonal and pandemic) in lung epithelial cells in vitro.
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| Overall design |
Well-differentiated primary human lung, bronchial epithelial cells (wd-NHBE) were infected with two pandemic and one seasaonl H1N1 influenza viruses at a multiplicity of infection of 3.0. The cells were then collected, in triplicate, at 36 hours after infection. This timepoint was selected as we saw significant differences in soluble cytokine and chemokine protein levels at this time point in the supernatant.
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| Contributor(s) |
Gerlach RL |
| Citation(s) |
24244384 |
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| Submission date |
Jul 02, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rachael Gerlach |
| E-mail(s) |
[email protected]
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| Organization name |
University of Louisville
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| Department |
Microbiology and Immunology
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| Lab |
Jonsson
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| Street address |
505 S Hancock, CTR 626
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| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM1179364 |
wdNHBE with BN/59 at 36hpi, biological rep1 |
| GSM1179365 |
wdNHBE with BN/59 at 36hpi, biological rep2 |
| GSM1179366 |
wdNHBE with BN/59 at 36hpi, biological rep3 |
| GSM1179367 |
wdNHBE with KY/136 at 36hpi, biological rep1 |
| GSM1179368 |
wdNHBE with KY/136 at 36hpi, biological rep2 |
| GSM1179369 |
wdNHBE with KY/136 at 36hpi, biological rep3 |
| GSM1179370 |
wdNHBE with KY/180 at 36hpi, biological rep1 |
| GSM1179371 |
wdNHBE with KY/180 at 36hpi, biological rep2 |
| GSM1179372 |
wdNHBE with KY/180 at 36hpi, biological rep3 |
| GSM1179373 |
wdNHBE uninfected at 36hpi, biological rep1 |
| GSM1179374 |
wdNHBE uninfected at 36hpi, biological rep2 |
| GSM1179375 |
wdNHBE uninfected at 36hpi, biological rep3 |
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| Relations |
| BioProject |
PRJNA210252 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48466_RAW.tar |
57.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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