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| Status |
Public on Dec 01, 2013 |
| Title |
Gene expression profiles of mammary epithelial and breast cancer cells following fever range hyperthermia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Preclinical and clinical studies have shown for decades that tumor cells demonstrate significantly enhanced sensitivity to “fever range” hyperthermia (increasing the intratumoral temperature to 42-45oC) than normal cells, although it is unknown why cancer cells exhibit this distinctive susceptibility. To address this issue, mammary epithelial cells and three malignant breast cancer lines were subjected to hyperthermic shock and microarray analysis of the global transcription changes was subsequently performed.
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| Overall design |
MCF10A (mammary epithelial cells) and MCF7, MDA231, and MDA468 breast cancer cells were grow at normal growth temperatures or subjected to 30 minutes of hyperthermic (45oC) shock followed by replacement with conditioned media at normal growth temperatures. RNA was collected 4 hours after shock and subjected to microarray analysis.
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| Contributor(s) |
Bryan BA, Mitchell DC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 28, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Brad Allen Bryan |
| E-mail(s) |
[email protected]
|
| Organization name |
Texas Tech University Health Sciences Center
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| Department |
Biomedical Sciences
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| Street address |
5001 El Paso Drive
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| City |
El Paso |
| State/province |
Texas |
| ZIP/Postal code |
79905 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA209945 |
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