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| Status |
Public on Jul 31, 2013 |
| Title |
SHAE003: SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HAE cultures were infected with SARS-CoV, SARS-ddORF6 or SARS-BatSRBD and were directly compared to A/CA/04/2009 H1N1 influenza-infected cultures. Cell samples were collected at various hours post-infection for analysis.
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| Overall design |
Time Points = 0, 12, 24, 36, 48, 60, 72, 84 and 96 hrs post-infection for SARS-CoV. Time Points = 0, 24, 48, 60, 72, 84 and 96 hrs post-infection forSARS-ddORF6 and SARS-BatSRBD. Time Points = 0, 6, 12, 18, 24, 36 and 48 hrs post-infection for H1N1. Done in triplicate/quadruplicate for RNA Triplicates/quadruplicates are defined as 3/4 different wells, plated at the same time and using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections. Infection was done at an MOI of 2.
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| Contributor(s) |
Baric R, Katze M, Sims A, Law GL, Chang J |
| Citation(s) |
23935999 |
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| Submission date |
Jun 14, 2013 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Michael Katze |
| E-mail(s) |
[email protected]
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| Organization name |
University of Washington
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| Department |
Microbiology
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| Lab |
Michael G. Katze, Ph.D
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| Street address |
Rosen Building 960 Republican St.
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (141)
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| This SubSeries is part of SuperSeries: |
| GSE47963 |
SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures. |
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| Relations |
| BioProject |
PRJNA208497 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47961_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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