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| Status |
Public on Jan 21, 2014 |
| Title |
Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 knockout (P4KO) and P4KO rescued with PMCA4a primary vascular smooth muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Husain lab cell proliferation in vitro experimental data showed that electroporating PMCA4a cDNA into P4KO cells rescued the G1 arrest phenotype in P4KO primary vascular smooth muscle cells (VSMC). This microarray documents the different gene expression profiles of P4KO and P4KO+PMCA4a VSMC at the late G1/S stage in serum synchronized cell populations (n=4 populations for each genotype).
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| Overall design |
Serum synchronized late G1/S stage cell populations (P4KO and P4KO+PMCA4a) were employed to extract total RNA which was used for microarray analysis.
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| Contributor(s) |
Afroze T, Husain M |
| Citation(s) |
24448801 |
| |
| Submission date |
May 08, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Mansoor Husain |
| E-mail(s) |
[email protected]
|
| Phone |
416-581-7489
|
| Organization name |
University Health Network
|
| Department |
Toronto Gen. Hosp. Res Institute
|
| Lab |
Mansoor Husain lab
|
| Street address |
Room TMDT-3-910, 101 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE38320 |
Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells |
|
| Relations |
| BioProject |
PRJNA202227 |
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