NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE45686 Query DataSets for GSE45686
Status Public on Mar 04, 2014
Title An extracellular matrix-driven positive feedback loop regulates translation in Idiopathic Pulmonary Fibrosis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Analysis of gene expression of lung fibroblasts seeded onto decellularized extracellular matrix (ECM). Experiment had 2x2 design where fibroblasts from idiopathic pulmonary fibrosis (IPF) or control patients were seeded onto decelluarized lung tissue from IPF or control patients allowing for determination of gene expression differences that were driven by IPF ECM and which differences were driven by the IPF fibroblast.
 
Overall design Lung fibroblasts from 5 patients with idiopathic pulmonary fibrosis and 5 control patients were cultured on decellularized ECM from IPF or control lung. Total RNA and polyribosome RNA were isolated after the cells were cultured on the decellularized ECM for 18 hours.
When possible, a control cell line and a diseased cell line were cultured (and processed) simultaneously to minimize the effect of experimental variance induced by running the experiment at different times.Samples with the same batch number (provied in the sample 'characteristics' field) were cultured and processed at the same time.
 
Contributor(s) Parker MW, Rossi D, Peterson MS, Sikstrom K, White ES, Henke C, Larsson O, Bitterman PB
Citation(s) 24590289
Submission date Apr 01, 2013
Last update date Aug 13, 2018
Contact name Matthew Parker
E-mail(s) [email protected]
Organization name University of Minnesota
Department Department of Medicine
Street address 420 Delaware St. SE
City Minneaplois
State/province MINNESOTA
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (40)
GSM1111915 heavy_control_controlECM_1
GSM1111916 total_control_controlECM_1
GSM1111917 heavy_control_IPFECM_1
Relations
BioProject PRJNA195605

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45686_RAW.tar 26.2 Mb (http)(custom) TAR
GSE45686_non_normalized.txt.gz 11.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap