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Series GSE4390

Query DataSets for GSE4390

Status

Public on Mar 09, 2006

Title

Analysis of expression in SOD1 transgenic mouse spinal cord

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

mRNA expression in the spinal cords of the G93A-SOD1 familial ALS transgenic mouse model was compared to that in nontransgenic (Normal mouse) and transgenic mice expressing wild-type (WT)SOD1. Gene Ontology (GO)analysis was used to characterize differences in expression between G93A-SOD1 mouse and nontransgenic mouse spinal cord. Changes in multiple GO categories were found. Many of these were associated with subsystems involving cell-cell communication and intracellular signal transduction. Expression profiles of mice expressing WT-SOD1 did not differ from nontransgenic mice. In contrast, protein profiling using proteomics technology indicated changes in mitochondrial protein expression in the G93A-SOD1 mouse spinal cord that were not found in the mRNA expression analysis.
Keywords: Disease state analysis, time course, transgenic mice

Overall design

Spinal cord mRNA expression was profiled using Affymetrix gene chips. 75-day old animals expressing G93A-SOD1 are presymptomatic, while 110-day animals display tremor and partial paralysis characteristic of ALS. WT-SOD1 animals exhibit no disease phenotype at 75 or 110 days. Nontransgenic mice at 75 and 110 days served as controls.

Contributor(s)

Lukas TJ, Luo W, Siddique T, Mao H, Cole N

Citation(s)

16571896

Submission date

Mar 07, 2006

Last update date

Feb 18, 2018

Contact name

Thomas J Lukas

E-mail(s)

[email protected]