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| Status |
Public on Feb 01, 2013 |
| Title |
Gene expression profiles of Tet1 knockout (T1KO), Tet2 knockout (T2KO) and Tet1/Tet2 double knockout (DKO) mouse embryonic stem cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Global gene expression profile of single and double mutant mouse ES cells were compared to wt ES cells. Two male Tet1 KO, one male Tet2 KO, two male double KO, two female double KO, two male WT and two female WT mouse ES cells were compared.
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| Overall design |
Global gene expression profile of single and double knockout mouse embryonic stem cells were compared to that of wild type mouse ES cells. All used ES lines were derived from C57/BL/6 mixed background mice. RNA from feeder free mutant mouse ES cells was competetively hybridized against RNA from WT ES cells. Same sex lines were compared. Two independent ES line of each genotype were used, with the exception of Tet2 KO ES cells where only one male line was used.
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| Contributor(s) |
Barrasa MI, Dawlaty MM, Jaenisch R |
| Citation(s) |
23352810 |
| |
| Submission date |
Dec 18, 2012 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Inmaculada Barrasa |
| E-mail(s) |
[email protected]
|
| Organization name |
Whitehead Institute
|
| Street address |
Nine Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (10)
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| GSM1054583 |
male DKO#51 versus male WT#6 Replicate 1 |
| GSM1054584 |
male DKO#51 versus male WT#6 Replicate 2 |
| GSM1054585 |
male DKO#61 versus male WT#92 Replicate 1 |
| GSM1054586 |
female DKO#60 versus female WT#7 Replicate 1 |
| GSM1054587 |
female DKO#59 versus female WT#94 Replicate 1 |
| GSM1054588 |
female DKO#59 versus female WT#94 Replicate 2 |
| GSM1054589 |
male T1KO#99 Replicate 1 |
| GSM1054590 |
male T1KO#99 Replicate 2 |
| GSM1054591 |
male T1KO#65versus male T2KO#16 Replicate 1 |
| GSM1054592 |
male T1KO#65versus male T2KO#16 Replicate 2 |
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| Relations |
| BioProject |
PRJNA184026 |
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