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| Status |
Public on Jun 30, 2013 |
| Title |
The microRNA cluster miR-17~92 promotes TFH cell differentiation and represses subset-inappropriate gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Follicular helper T cells (TFH cells) are the prototypic helper T cell subset specialized to enable B cells to form germinal centers (GCs) and produce high-affinity antibodies. We found that expression of microRNAs (miRNAs) by T cells was essential for TFH cell differentiation. More specifically, we show that after immunization of mice with protein, the miRNA cluster miR-17~92 was critical for robust differentiation and function of TFH cells in a cell-intrinsic manner that occurred regardless of changes in proliferation. In a viral infection model, miR-17~92 restrained the expression of genes ‘inappropriate’ to the TFH cell subset, including the direct miR-17~92 target Rora. Removal of one Rora allele partially ‘rescued’ the inappropriate gene signature in miR-17~92-deficient TFH cells. Our results identify the miR-17~92 cluster as a critical regulator of T cell–dependent antibody responses, TFH cell differentiation and the fidelity of the TFH cell gene-expression program.
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| Overall design |
Gene expression analysis of control versus miR-17~92 knockout (KO) LCMV-specific SMARTA TFH cells 5.5 days after viral infection.
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| Contributor(s) |
Baumjohann D, Kageyama R, Jeker LT, Ansel KM |
| Citation(s) |
23812098 |
| |
| Submission date |
Dec 05, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Dirk Baumjohann |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Bonn
|
| Department |
Medical Clinic III
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| Lab |
Baumjohann Lab
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| Street address |
Venusberg-Campus 1
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| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53127 |
| Country |
Germany |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA183070 |
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