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| Status |
Public on Nov 19, 2013 |
| Title |
Integrated analysis of transcript level regulation of metabolism during human adipocyte differentiation [miR] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Here, we have focused on studying the link between metabolic changes driven by the differentiation into mature adipocytes of a human preadipocyte cell line (SGBS) and their regulation, through a combined experimental and computational approach.
By collecting data on gene expression, PPARg, CEBPa, LXR and H3K4me3 genome-wide ChIP-seq profles and transcriptome-wide microRNA target identification for miR-27a, miR29a and miR-222, and using constraint-based modeling to estimate metabolic reaction activity, we obtained a comprehensive set of information highlighting how epigenetic, transcriptional and post-transcriptional regulation impacts the metabolic network.
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| Overall design |
Illumina HT12 V4.0 microarrays: Transcriptome analysis 24 hours following overexpression of indicated microRNAs or control siRNAs on indicated time points of differentiation (D0 or D4 + 24 hours) (7 conditions in triplicate, total of 21 samples + siLamin transfection as positive control in duplicate)
This submission represents transcriptome component of study.
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| |
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| Contributor(s) |
Heinäniemi M |
| Citation(s) |
24198249 |
| |
| Submission date |
Oct 15, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Merja Heinäniemi |
| Organization name |
university of eastern finland
|
| Street address |
Yliopistonranta 1
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (23)
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| This SubSeries is part of SuperSeries: |
| GSE41578 |
Integrated analysis of transcript level regulation of metabolism during human adipocyte differentiation |
|
| Relations |
| BioProject |
PRJNA177564 |
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