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| Status |
Public on Sep 28, 2012 |
| Title |
Gene expression analysis of peripheral blood mononuclear cells prior to and following vaccination of human volunteers with the novel TB vaccine candidate MVA85A |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A greater understanding of the relationships between a vaccine, the immune response it induces, and protection from disease would greatly facilitate vaccine development. Modified Vaccinia virus Ankara expressing antigen 85A (MVA85A) is a novel tuberculosis (TB) vaccine designed to enhance responses induced by Bacille Calmette-Guerin (BCG). Antigen-specific interferon-γ (IFN-γ) production is greatly enhanced by MVA85A and peaks one week post-vaccination, however, the variability in response between healthy individuals is extensive. In this study we have sought to characterize the early changes in gene expression following vaccination with MVA85A and understand how these are related to long-term immunogenicity. 24 volunteers were vaccinated with 10^8 pfu MVA85A. 12 volunteers were vaccinated intramuscularly and 12 intradermally. Volunteers were healthy and did not have HIV, HCV, HBV or latent or active tuberculosis. Blood for this study was taken on the day of vaccination, immediately prior to the vaccine being given (day 0), and 2 and 7 days post-vaccination.
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| Overall design |
Volunteers with a prior BCG vaccination were vaccinated with 1x10^8 pfu MVA85A either intradermally (id) or intramuscularly (im). Blood was taken immediately prior to vaccination (day 0) and 2 and 7 days post-vaccination. PBMCs were separated and frozen down. PBMCs were then thawed and RNA was extracted directly for microarray analysis. Some arrays contain control samples: from volunteers, incubated with either media alone or antigen 85, MVA85A or MVA wild type. These samples were used separately.
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| Contributor(s) |
Matsumiya M |
| Citation(s) |
23844129, 24912498 |
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| Submission date |
Sep 09, 2012 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Magali Matsumiya |
| E-mail(s) |
[email protected]
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| Organization name |
Jenner Institute, University of Oxford
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| Street address |
Roosevelt Drive
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| City |
Oxford |
| ZIP/Postal code |
OX37DQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (96)
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| GSM999677 |
vol11 pbmc day 0, rep 1 |
| GSM999678 |
vol11 pbmc day 2, rep 1 |
| GSM999679 |
vol11 pbmc day 7, rep 1 |
| GSM999680 |
vol20 pbmc day 0, rep 1 |
| GSM999681 |
vol20 pbmc day 2, rep 1 |
| GSM999682 |
vol20 pbmc day 7, rep 1 |
| GSM999683 |
vol16 6hr media in vitro |
| GSM999684 |
vol16 6hr mva85a in vitro |
| GSM999685 |
vol24 pbmc day 2 rep 2 |
| GSM999686 |
vol2 pbmc day 0, rep 2 |
| GSM999687 |
vol2 pbmc day 2, rep 1 |
| GSM999688 |
vol2 pbmc day 7, rep 1 |
| GSM999689 |
vol12 pbmc day 0, rep 1 |
| GSM999690 |
vol12 pbmc day 2, rep 1 |
| GSM999691 |
vol12 pbmc day 7, rep 1 |
| GSM999692 |
vol21 pbmc day 0, rep 1 |
| GSM999693 |
vol21 pbmc day 2, rep 1 |
| GSM999694 |
vol21 pbmc day 7, rep 1 |
| GSM999695 |
vol17 6hr media in vitro |
| GSM999696 |
vol 17 6hr mva85a in vitro |
| GSM999697 |
vol24 pbmc day 7, rep 2 |
| GSM999698 |
vol3 pbmc day 0, rep 1 |
| GSM999699 |
vol3 pbmc day 2, rep 1 |
| GSM999700 |
vol3 pbmc day 7, rep 1 |
| GSM999701 |
vol13 pbmc day 0, rep 1 |
| GSM999702 |
vol13 pbmc day 2, rep 1 |
| GSM999703 |
vol13 pbmc day 7, rep 1 |
| GSM999704 |
vol22 pbmc day 0, rep 1 |
| GSM999705 |
vol22 pbmc day 2, rep 1 |
| GSM999706 |
vol22 pbmc day 7, rep 1 |
| GSM999707 |
vol26 6hr media in vitro |
| GSM999708 |
vol26 6hr mva85a in vitro |
| GSM999709 |
vol29 pbmc day 2, rep 2 |
| GSM999710 |
vol5 pbmc day 0, rep 1 |
| GSM999711 |
vol5 pbmc day 2, rep 1 |
| GSM999712 |
vol5 pbmc day 7, rep 1 |
| GSM999713 |
vol14 pbmc day 0, rep 1 |
| GSM999714 |
vol14 pbmc day 2, rep 1 |
| GSM999715 |
vol14 pbmc day 7, rep 1 |
| GSM999716 |
vol24 pbmc day 0, rep 1 |
| GSM999717 |
vol24 pbmc day 2, rep 1 |
| GSM999718 |
vol24 pbmc day 7, rep 1 |
| GSM999719 |
vol28 6hr media in vitro |
| GSM999720 |
vol28 6hr mva85a in vitro |
| GSM999721 |
vol29 pbmc day 7, rep 2 |
| GSM999722 |
vol6 pbmc day 0, rep 1 |
| GSM999723 |
vol6 pbmc day 2, rep 1 |
| GSM999724 |
vol6 pbmc day 7, rep 1 |
| GSM999725 |
vol15 pbmc day 0, rep 1 |
| GSM999726 |
vol15 pbmc day 2, rep 1 |
| GSM999727 |
vol15 pbmc day 7, rep 1 |
| GSM999728 |
vol25 pbmc day 0, rep 1 |
| GSM999729 |
vol25 pbmc day 2, rep 1 |
| GSM999730 |
vol25 pbmc day 7, rep 1 |
| GSM999731 |
vol16 6hr 85a in vitro |
| GSM999732 |
vol16 6hr mvawt in vitro |
| GSM999733 |
vol12 pbmc day 2, rep 2 |
| GSM999734 |
vol7 pbmc day 0, rep 1 |
| GSM999735 |
vol7 pbmc day 2, rep 1 |
| GSM999736 |
vol7 pbmc day 7, rep 1 |
| GSM999737 |
vol16 pbmc day 0, rep 1 |
| GSM999738 |
vol16 pbmc day 2, rep 1 |
| GSM999739 |
vol16 pbmc day 7, rep 1 |
| GSM999740 |
vol26 pbmc day 0, rep 1 |
| GSM999741 |
vol26 pbmc day 2, rep 1 |
| GSM999742 |
vol26 pbmc day 7, rep 1 |
| GSM999743 |
vol17 6hr 85a in vitro |
| GSM999744 |
vol17 6hr mvawt in vitro |
| GSM999745 |
vol12 pbmc day 7, rep 2 |
| GSM999746 |
vol9 pbmc day 0, rep 1 |
| GSM999747 |
vol9 pbmc day 2, rep 1 |
| GSM999748 |
vol9 pbmc day 7, rep 1 |
| GSM999749 |
vol17 pbmc day 0, rep 1 |
| GSM999750 |
vol17 pbmc day 2, rep 1 |
| GSM999751 |
vol17 pbmc day 7, rep 1 |
| GSM999752 |
vol28 pbmc day 0, rep 1 |
| GSM999753 |
vol28 pbmc day 2, rep 1 |
| GSM999754 |
vol28 pbmc day 7, rep 1 |
| GSM999755 |
vol26 6hr 85a in vitro |
| GSM999756 |
vol26 6hr mvawt in vitro |
| GSM999757 |
vol28 pbmc day 2, rep 2 |
| GSM999758 |
vol10 pbmc day 0, rep 1 |
| GSM999759 |
vol10 pbmc day 2, rep 1 |
| GSM999760 |
vol10 pbmc day 7, rep 1 |
| GSM999761 |
vol19 pbmc day 0, rep 1 |
| GSM999762 |
vol19 pbmc day 2, rep 1 |
| GSM999763 |
vol19 pbmc day 7, rep 1 |
| GSM999764 |
vol29 pbmc day 0, rep 1 |
| GSM999765 |
vol29 pbmc day 2, rep 1 |
| GSM999766 |
vol29 pbmc day 7, rep 1 |
| GSM999767 |
vol28 6hr 85a in vitro |
| GSM999768 |
vol28 6hr mvawt in vitro |
| GSM999769 |
vol28 pbmc day 7, rep 2 |
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| Relations |
| BioProject |
PRJNA174748 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40719_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE40719_non-normalized.txt.gz |
27.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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