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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 03, 2012 |
| Title |
Expression data from mouse embryonic stem cells and ES cell-derived definitive endoderm and early hepatic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Foxa2 is required for endoderm differentiation into the hepatic lineage. The mechanism of activation for Foxa2 during this developmental process has not been elucidated yet. We established an in vitro system to guide ES cells differentiating into definitive endoderm (DE) cells and the following DE cells to early hepatic cells. ChIP-seq assays have been successfully performed to assess the Foxa2 binding profile. Over 50% of Foxa2 target genes in DE cells were found to be activated in hepatic cells which were at a stage later than the DE stage. Therefore, our finding at the genome-wide level proved Foxa2 serves as a pioneer factor at the DE stage. Furthermore, Foxa2 could specifically induce H3K4me2 modifications to the promoter/enhancer regions of many hepatic genes to pre-mark the chromatin, and determine the hepatic lineage differentiation competence. Our study illustrated the wide existence of Foxa2's pioneer factor function and uncovered the correlation between pioneer factor and chromatin pre-mark. These findings will be helpful for understanding the developmental process of hepatogenesis and efficiently controlling Foxa2 during hepatic induction for generating functional hepatocytes.
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| Overall design |
To further gain a genome-wide view of Foxa2's effects on its target gene expression, 3 representative time points from the ES cell differentiation process to hepatic cells were selected for cDNA microarray analysis: 1) Day 0, representing ES cells, where there was no Foxa2 expression; 2) Day 5, representing DE cells; and 3) Day 7, representing early hepatic cells.
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| Contributor(s) |
Xu C, Lyu X, Chen EZ, He Z, Uyunbilig B, Li G, Ma Y, Hui L, Xie B, Gao Y, Ding X, Hu Y, Hu P, Han JJ, Wang X |
| Citation(s) |
22750789 |
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| Submission date |
Aug 02, 2012 |
| Last update date |
Apr 04, 2017 |
| Contact name |
Chenhuan Xu |
| E-mail(s) |
[email protected]
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| Organization name |
Beijing Institute of Genomics
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| Street address |
1 Beichen West Road
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA171861 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39854_RAW.tar |
27.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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