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| Status |
Public on Jul 10, 2012 |
| Title |
The NS1 protein of influenza A virus suppresses interferon-regulated activation of antigen-presentation and immune-proteasome pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The NS1 protein of influenza virus counters host antiviral defences primarily by antagonizing the type I interferon (IFN) response. Both the N-terminal dsRNA-binding domain and the C-terminal effector domain are required for optimal suppression of host responses during infection. To better understand the regulatory role of the NS1 effector domain, we used an NS1-truncated mutant virus derived from human H1N1 influenza isolate A/Texas/36/91 (Tx/91) and assessed global transcriptional profiles from two independent human lung cell-culture models.
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| Overall design |
Primary human tracheobronchial epithelial (HTBE) cells were washed and infected with 4×10^6 p.f.u. Tx/91 or Tx/91 NS1 : 1–126 viruses per filter (m.o.i. of 20) for 1 h at 37 °C. Three replicate wells were infected for each condition per time point. Allantoic fluid was used for mock treatments. At 9.5 and 25 h p.i., cells were collected and resuspended in TRIzol reagent (Invitrogen) for total RNA extraction. A549 cells were infected with Tx/91 or Tx/91 NS1 : 1–126 viruses at an m.o.i. of 2 for 1 h at 4 °C. Three replicate wells were infected for each condition per time point. Allantoic fluid was used for mock treatments. A549 cells were collected and lysed in solution D (4 M guanidinium thiocyanate, 25 mM sodium citrate, 0.5 % sarcosyl, 0.1 M β-mercaptoethanol) at 12 and 24 h p.i. for total RNA extraction.
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| Contributor(s) |
Tisoncik JR, Billharz R, Burmakina S, Belisle SE, Proll SC, Korth MJ, Garcia-Sastre A, Katze MG |
| Citation missing |
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| |
| Submission date |
Jul 09, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Michael Katze |
| E-mail(s) |
[email protected]
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| Organization name |
University of Washington
|
| Department |
Microbiology
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| Lab |
Michael G. Katze, Ph.D
|
| Street address |
Rosen Building 960 Republican St.
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (2) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE39202 |
NS1 protein of influenza A virus and interferon |
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| Relations |
| BioProject |
PRJNA170197 |
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