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Series GSE3863

Query DataSets for GSE3863

Status

Public on Dec 31, 2005

Title

VFB mutant analysis

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Transcript profiling analysis of vfb (Vier F-Box) mutant seedlings compared to wild type using Arabidopsis ATH1 GeneChip array.
Keywords: 10 day old light grown seedlings, wild type and mutant

Overall design

Three replicate samples were harvested and prepared from 10 day-old light grown wild type and vfb mutant seedlings. RNA for micro array analysis was extracted using the RNeasyTM kit (Quiagen, Hilden, Germany). Complementary RNA was prepared from 6 µg of total RNA as described in Affimetrix _Expression Analysis Technical Manual using the One-Cycle Target Labeling and Control Reagents (Affimetrix, Santa Clara, CA). In brief, double-stranded cDNA was synthesized. Biotin-labeled target cRNA was prepared by cDNA in vitro transcription in the presence of biotinylated UTP and CTP. After purification, cRNA was fragmented and used to hybridize to the Arabidopsis ATH1 GeneChip array (Affimetrix, Santa Clara, CA). Hybridization, washing, staining, scanning and data collection were performed in an Affymetrix GeneChip® Fluidics Station 450 and GeneArray® Scanner. The microarray computaional analysis was performed on *.CEL and *.CHP data files

Contributor(s)

Calderon-Villalobos L

Citation(s)

17435085

Submission date

Dec 19, 2005

Last update date

Jun 12, 2017

Contact name

Claus Schwechheimer

E-mail(s)

[email protected]