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Series GSE3784

Query DataSets for GSE3784

Status

Public on Dec 10, 2005

Title

Seed germination in presence and absence of histone deaceatylase inhibitor, Trichostain A (TSA).

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes.
Keywords: histone deacetylase inbibition, developmental effects

Overall design

Three samples: Unimbibed seeds, untreated imbibed seeds, TSA-treated imbibed seeds.

Contributor(s)

Tai HH, Tai GC, Beardmore T

Citation(s)

16307366

Submission date

Dec 08, 2005

Last update date

Jun 12, 2017

Contact name

Helen H Tai