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| Status |
Public on May 05, 2012 |
| Title |
Analysis of differential genetic expression by moderate-carbohydrate restriction diet with calorie restriction mimetic multiple phytochemicals (MCDmp) using a transgenic liver cancer model developed by a simple hydrodynamic transfection method. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We performed survival analysis of control and MCDmp groups, and explored underlying tumor suppression mechanisms after dietary intervention, focused on alterations in the energy-dependent signaling pathways, and global gene expression differences on cDNA microarray study.
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| Overall design |
Five- week- old male C57BL6 mice were randomly divided into two groups and fed control diet (control group, LabDiet, Brentwood, MO, USA) or MCDmp formula (Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee. Following a preliminary feeding of each diet formula for two week, HrasG12V / shp53 / GFP4 gene containing transposon vector were injected into mouse tail vein by hydrodynamic injection method. After 4 weeks of diet supplementation, all mice were sacrificed. Mouse liver tissue was excised for microarray analysis.
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| Contributor(s) |
Lee JD, Choi M, Ro WS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 04, 2012 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Jong-Doo Lee |
| E-mail(s) |
[email protected]
|
| Phone |
82-10-2829-1159
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| Fax |
82-2-312-0578
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| Organization name |
Yonsei University
|
| Street address |
250 seonsan-ro seodaemum-gu
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| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| GSM927335 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 1 |
| GSM927336 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 2 |
| GSM927337 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 3 |
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| Relations |
| BioProject |
PRJNA163323 |
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