|
| Status |
Public on Oct 16, 2012 |
| Title |
Gene expression data from glioblastoma tumor samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Glioblastoma (GBM) is an incurable brain tumor carrying a dismal prognosis, which displays considerable heterogeneity. We have recently identified recurrent H3F3A mutations affecting two critical positions of histone H3.3 (K27, G34) in one-third of pediatric GBM. Here we show that each of these H3F3A mutations defines an epigenetic subgroup of GBM with a distinct global methylation pattern, and are mutually exclusive with IDH1 mutation (characterizing a CpG-Island Methylator Phenotype (CIMP) subgroup). Three further epigenetic subgroups were enriched for hallmark genetic events of adult GBM (EGFR amplification, CDKN2A/B deletion) and/or known transcriptomic signatures. We also demonstrate that the two H3F3A mutations give rise to GBMs in separate anatomic compartments, with differential regulation of OLIG1/2 and FOXG1, possibly reflecting different cellular origins.
To further dissect the biological differences between epigenetic glioblastoma subgroups, we looked at the transcriptomic profiles of glioblastoma samples.
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| Overall design |
46 glioblastoma samples from patients of various ages were selected for RNA extraction and hybridization on Affymetrix Affymetrix Human Genome U133 Plus 2.0 Arrays.
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| Contributor(s) |
Sturm D, Witt H, Hovestadt V, Khuong Quang D, Plass C, Jabado N, Pfister SM |
| Citation(s) |
23079654 |
| |
| Submission date |
Mar 02, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Marcel Kool |
| E-mail(s) |
[email protected]
|
| Phone |
00496221424636
|
| Organization name |
German Cancer Research Center
|
| Department |
Pediatric Neuro Oncology
|
| Street address |
Im Neuenheimer Feld 580
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (46)
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| Relations |
| BioProject |
PRJNA153165 |
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