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| Status |
Public on Apr 28, 2012 |
| Title |
Identification of a complex genetic network involved in Saccharomyces cerevisiae colony morphology |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
When grown on solid substrates, different microorganisms often form colonies with very specific morphologies. Whereas the pioneers of microbiology often used colony morphology to discriminate between species and strains, the phenomenon has not received much recent attention. In this study, we use a genome-wide assay in the model yeast Saccharomyces cerevisiae to identify all genes that affect colony morphology. We show that several major signaling cascades, including the MAPK, TORC, SNF1 and RIM101 pathways play a role, indicating that morphological changes are a reaction to changing environments. Other genes that affect colony morphology are involved in protein sorting and epigenetic regulation. Interestingly, the screen reveals only few genes that are likely to play a direct role in establishing colony morphology, one notable exception being FLO11, a gene encoding a cell-surface adhesin that has already been implicated in colony morphology, biofilm formation, and invasive and pseudohyphal growth. Using a series of modified promoters to tune FLO11 expression, we confirm the central role of Flo11 and show that differences in FLO11 expression result in distinct colony morphologies. Together, our results provide a first comprehensive looks at the complex genetic network that underlies the diversity in the morphologies of yeast colonies.
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| Overall design |
Microarrays were used to measure gene expression between WT and FLO11 mutants in both liquid and solid medium to assess which genes induce altered colony morphology through FLO11.
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| Contributor(s) |
van der Zande E, De Maeyer D, Verstrepen K |
| Citation(s) |
22882838 |
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| Submission date |
Feb 29, 2012 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Dries De Maeyer |
| Organization name |
CMPG/Bioincubator
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| Street address |
Gaston Geenslaan 1
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| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA162443 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36151_RAW.tar |
16.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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