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Series GSE35386 Query DataSets for GSE35386
Status Public on Oct 31, 2014
Title GENE EXPRESSION CHANGES WITHIN MÜLLER GLIAL CELLS DURING RETINITIS PIGMENTOSA
Organism Mus musculus
Experiment type Expression profiling by array
Summary Retinitis Pigmentosa (RP) is a progressive retinal degeneration in which the retina loses nearly all of its photoreceptor cells and undergoes major structural changes. Little is known regarding the role the resident glia, the Müller glia, play in the progression of the disease. Here we define gene expression changes in Müller glial cells (MGCs) from two different mouse models of RP, the retinal degeneration 1 (rd1) and rhodopsin knock-out (Rhod-ko) models. The RNA repertoire of 28 single MGCs was comprehensively profiled, and a comparison was made between MGC from wild type (WT) and mutant retinas. Two time points were chosen for analysis, one at the peak of rod photoreceptor death and one during the period of cone photoreceptor death. MGCs have been shown to respond to retinal degeneration by undergoing gliosis, a process marked by the upregulation of GFAP. In this data, many additional transcripts were found to change. These can be placed into functional clusters, such as retinal remodeling, stress response, and immune related response. It is noteworthy that a high degree of heterogeneity among the individual cells was observed, possibly due to their different spatial proximities to dying cells, and/or inherent heterogeneity among MGCs.
 
Overall design Retinas were dissociated and single Muller Glial Cells were picked under a dissecting microscope using a micropipette based on their distinct morphological shape. Single cell cDNAs were generated and genome wide profiles were obtained by hybridization to Affymetrix 430 2.0 microarrays. Data was normalized using MAS5.0 software. A total of 28 Muller Glial Cells were analyzed and confirmed post hoc by expression of known marker genes.
 
Contributor(s) Roesch K, Trimarchi JM, Cepko CL
Citation(s) 22665967
Submission date Jan 27, 2012
Last update date Feb 11, 2019
Contact name Karin Roesch
Organization name Harvard Medical School
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (28)
GSM867192 single cell profile of retinal Muller Glial Cell WT MG1
GSM867193 single cell profile of retinal Muller Glial Cell WT MG16
GSM867194 single cell profile of retinal Muller Glial Cell WT MG22
Relations
BioProject PRJNA152627

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Supplementary file Size Download File type/resource
GSE35386_RAW.tar 94.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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