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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 20, 2012 |
| Title |
Determination of the protein interactome of the transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Coordinate expression of the somatic cell reprogramming factors Oct4, Sox2, Klf4 and c-Myc within embryonic stem cells preserves the self-renewal of these cells, while allowing for the expression epitope tagged Sox2. Taking advantage of this observation, we engineered embryonic stem cells (i-OSKM-ESC) to inducibly express Oct4, Klf4, c-Myc and an epitope tagged form of Sox2 from a polycistronic element, in the presence of doxycycline. We isolated Sox2 and its associated protein complexes by co-immunoprecipitation. Subsequently, we identified the Sox2-protein interactome in self-renewing embryonic stem cells using an unbiased proteomic screen (Multidimensional Protein Identification Technology [MudPIT]).
Affymetrix microarrays were used to characterize the gene expression profile of i-OSKM-ESC in the absence and presence of doxycycline.
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| Overall design |
Mouse embryonic stem cells (KH2) were engineered to express Oct4, epitope tagged Sox2, Klf4 and c-Myc in the presence of doxycyline, to produce i-OSKM-ESC. The i-OSKM-ESC were cultured in the absence or presence of doxycyline (4 µg/mL) for 24 hours. RNA was extracted from each condition, and used for microarray analysis.
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| Contributor(s) |
Gao Z, Cox JL, Gilmore JM, Ormsbee BD, Mallanna SK, Washburn MP, Rizzino A |
| Citation(s) |
22334693 |
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| Submission date |
Jan 02, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Angie Rizzinio |
| E-mail(s) |
[email protected]
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| Organization name |
University of Nebraska Medical Center
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| Department |
Eppley Institute for Research in Cancer and Allied Diseases
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| Street address |
986805 Nebraska Medical Center
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| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198-6805 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA151129 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34801_RAW.tar |
8.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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