|
| Status |
Public on Oct 02, 2012 |
| Title |
Cigarette smoking alters mRNA expression in human alveolar macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Alveolar macrophages from never smokers and active smokers were isolated by bronchoalveolar lavage and gene expression was measured. Chronic cigarette smoke exposure, as occurs in smoker's lungs, leads to significant changes in gene expression. Of note, RNA was isolated immediately following bronchoscopy. Alveolar macrophage levels were >95%.
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| |
|
| Overall design |
4 never smokers and 4 active smokers were recruited for the study. After bronchoalveolar lavage and RNA isolation, gene expression was measured using Affymetrix Human Exon 1.0 ST arrays.
|
| |
|
| Contributor(s) |
Monick MM, Bair T |
| Citation(s) |
22952876, 24668803 |
| |
| Submission date |
Dec 16, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Thomas B Bair |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Iowa
|
| Street address |
335 EMRB
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA151409 |
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