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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 01, 2011 |
| Title |
Expression in irradiated MEFs exposed to murine acute lymphoblastic leukemia cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Primary pre-B acute lymphoblastic (ALL) cells do not proliferate long-term ex vivo without the presence of stromal support. We developed and use an ex vivo co-culture model, consisting of mouse leukemic pre-B Bcr/Abl-expressing ALL cells grown with mitotically inactivated mouse embryonic fibroblasts (MEFs). This system provides a generic type of environmentally-mediated protection to the ALL cells, because when the ALL cells are treated with a moderate dose of a therapeutic drug, drug-resistant ALL cells can be recovered after a 1-2 week period of culture. Some of the factors produced by stromal cells that provide protection to ALL cells have been identified. However, it is unclear if the presence of drug-treated ALL cells affects the stromal fibroblasts. The current study was initiated to examine this using expression profiling on the irradiated MEFs.
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| Overall design |
We isolated RNA from irradiated wild-type MEFs after a 9-day culture period. MEFs had either been exposed to DMSO, to nilotinib, a drug that is not expected to affect them, or to ALL cells treated with DMSO or nilotinib. MEFs and ALL cells were physically separated by a Transwell membrane of a pore size that did not allow the ALL cells to migrate towards the MEFs, but allowed the exchange of diffusible molecules.
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| Contributor(s) |
Heisterkamp N, Stoddard S, Feldhahn N, Arutyunyan A |
| Citation(s) |
22916712 |
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| Submission date |
Oct 30, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Nora Heisterkamp |
| E-mail(s) |
[email protected]
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| Phone |
626-218-7503
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| Organization name |
Beckman Research Institute City of Hope
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| Department |
Department of Systems Biology
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| Street address |
1218 S Fifth Avenue
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| City |
Monrovia |
| State/province |
CA |
| ZIP/Postal code |
91016 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM824258 |
irradiated MEFs exposed to DMSO-treated ALL cells |
| GSM824259 |
irradiated MEFs exposed to nilotinib-treated ALL cells, biological replicate 1 |
| GSM824260 |
irradiated MEFs exposed to nilotinib-treated ALL cells, biological replicate 2 |
| GSM824261 |
irradiated MEFs exposed to nilotinib-treated ALL cells, biological replicate 3 |
| GSM824262 |
irradiated MEFs exposed to DMSO, biologcal replicate 1 |
| GSM824263 |
irradiated MEFs exposed to DMSO, biologcal replicate 2 |
| GSM824264 |
irradiated MEFs exposed to DMSO, biologcal replicate 3 |
| GSM824265 |
irradiated MEFs exposed to nilotinib, biological replicate 1 |
| GSM824266 |
irradiated MEFs exposed to nilotinib, biological replicate 2 |
| GSM824267 |
irradiated MEFs exposed to nilotinib, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA148995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33329_RAW.tar |
46.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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