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| Status |
Public on Nov 22, 2011 |
| Title |
Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Long term exposure to incretin hormones is known to have salutory effects on beta cell function and viability. While short-term cAMP induction is known to have a signature CREB-CRTC target gene response, the long-term effects of cAMP on beta cell gene expression are less well understood.
We used rat microarray analysis to compare the genome-wide gene expression response to short-term (2 hours) and long-term (16 hours) stimulations of the cAMP agonist forskolin in INS-1 insulinoma cells.
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| Overall design |
INS-1 cells were exposed to forskolin for 2 or 16 hours in RPMI medium containing 10% serum. Control samples wer incubated for the same time without forskolin. Extracted RNA was used for hybridization on Affymetrix rat 1.0 st gene arrays.
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| Contributor(s) |
Van de Velde S |
| Citation(s) |
21949366 |
| |
| Submission date |
Aug 30, 2011 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Sam Van de Velde |
| E-mail(s) |
[email protected]
|
| Organization name |
Salk Institute
|
| Department |
Peptide Biology Laboratories
|
| Lab |
Montminy
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| Street address |
10010 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA145433 |
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