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| Status |
Public on Aug 15, 2011 |
| Title |
Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Here, we analyzed small RNA libraries derived from ovarian tissues heterozygous or mutant for the Tudor gene, Vreteno. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline, piRNAs can engage in an Aub/Argonaute 3 (AGO3)-dependent amplification in the absence of Vret, suggesting that Vret function can distinguish between primary piRNAs loaded into Piwi/Aub complexes and piRNAs engaged in the amplification cycle. We propose that Vret acts at an early step in primary piRNA processing where it plays an essential role in transposon regulation.
Keyword : Epigenetics
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| Overall design |
2 libraries were analyzed, with 1 being a control (heterozygote).
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| Contributor(s) |
Malone CD, Zamparini AL, Lehmann R |
| Citation(s) |
21831924 |
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| Submission date |
Jun 20, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Colin Dean Malone |
| E-mail(s) |
[email protected]
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| Phone |
708-602-2170
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| Organization name |
NYU Medical Center
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| Department |
Skirball Institute - Developmental Genetics
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| Lab |
Lehmann Lab
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| Street address |
NYUMC, Skirball Institute, 540 1st Ave, 4-10
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL9061 |
Illumina Genome Analyzer II (Drosophila melanogaster) |
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| Samples (2) |
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| Relations |
| SRA |
SRP007330 |
| BioProject |
PRJNA143881 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30088_RAW.tar |
26.7 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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