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| Status |
Public on May 04, 2011 |
| Title |
Gene expression profiling of high (HAB) vs. low (LAB) and normal (NAB) anxiety-related behavior mice in five laser microdissected brain regions. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Investigating the molecular basis and correlates of anxiety-related and depression-like behaviors, we generated a mouse model consisting of high (HAB), normal (NAB) and low (LAB) anxiety-related behavior mice. We utilized the elevated plus-maze for testing the genetic predisposition to anxiety-related behavior and, consequently, used this as selection criterion for the inbreeding of our animals. In depression-related tests, HAB mice display a more passive, depression-like coping strategy than LAB mice, resembling clinical comorbidity of anxiety and depression as observed in psychiatric patients.
Using a microarray approach, the hypothalamic paraventricular nucleus (PVN), the basolateral (BLA) and central amygdala (CeA), the cingulate cortex (Cg) and the dentate gyrus (DG) – centers of the central nervous anxiety and fear circuitries – were investigated and screened for differences between HAB, NAB and LAB mice.
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| Overall design |
Analysis was performed from four to six animals per line (HAB, NAB and LAB from generation 25, respectively) per brain region, giving a total of 78 individual arrays analyzed. The LAB mouse line is referred to as reference.
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| Contributor(s) |
Czibere L, Weber P, Pütz B, Kühne C, Rieder G, Holsboer F, Deussing JM, Landgraf R |
| Citation(s) |
21897848 |
| |
| Submission date |
May 02, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Ludwig Czibere |
| E-mail(s) |
[email protected]
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| Organization name |
Max Planck Institute of Psychiatry
|
| Street address |
Kraepelinstrasse 2
|
| City |
München |
| ZIP/Postal code |
80804 |
| Country |
Germany |
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| Platforms (1) |
| GPL6481 |
Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version] |
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| Samples (78)
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| This SubSeries is part of SuperSeries: |
| GSE29015 |
Gene expression analyses of mice selectively inbred for trait anxiety |
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| Relations |
| BioProject |
PRJNA143119 |
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