|
| Status |
Public on Jul 25, 2012 |
| Title |
Histone exchange and histone H4 acetylation in wild-type and SET2 deletion yeast strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
ChIP-on chip assays to measure the change in histone exchange or histone acetylation over the yeast genome, in a SET2 deleted strain compared to the wild-type control. ChIP of Flag and Acetylated H4 from wild-type and SET2 deleted cells were normalized to the Myc enrichment.
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| |
|
| Overall design |
Two color experiment. SET2 deletion vs. WT cells. Biological replicates=3 per IP per cell type.
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| |
|
| Contributor(s) |
Venkatesh S, Li H, Workman JL |
| Citation(s) |
22914091 |
| |
| Submission date |
Mar 22, 2011 |
| Last update date |
Sep 07, 2013 |
| Contact name |
Swaminathan Venkatesh |
| E-mail(s) |
[email protected]
|
| Phone |
816-926-4312
|
| Fax |
816-926-4866
|
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Workman Lab
|
| Street address |
1000 E 50th Street
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64112 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10930 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) [Probe Name version] |
|
| Samples (18)
|
| GSM696088 |
SET2 deletion Flag IP Replicate 1 (MDY510) |
| GSM696089 |
SET2 deletion Flag IP Replicate 2 (MDY510) |
| GSM696090 |
SET2 deletion Flag IP Replicate 3 (MDY510) |
| GSM696091 |
WT myc IP Replicate 1 (MDY510) |
| GSM696092 |
WT myc IP Replicate 2 (MDY510) |
| GSM696093 |
WT myc IP Replicate 3 (MDY510) |
| GSM696094 |
SET2 deletion myc IP Replicate 1 (MDY510) |
| GSM696095 |
SET2 deletion myc IP Replicate 2 (MDY510) |
| GSM696096 |
SET2 deletion myc IP Replicate 3 (MDY510) |
| GSM696097 |
WT AcH4 IP Replicate 1 (MDY510) |
| GSM696098 |
WT AcH4 IP Replicate 2 (MDY510) |
| GSM696099 |
WT AcH4 IP Replicate 3 (MDY510) |
| GSM696100 |
SET2 deletion AcH4 IP Replicate 1 (MDY510) |
| GSM696101 |
SET2 deletion AcH4 IP Replicate 2 (MDY510) |
| GSM696102 |
SET2 deletion AcH4 IP Replicate 3 (MDY510) |
|
| This SubSeries is part of SuperSeries: |
| GSE28099 |
Co-transcriptional histone acetylation is a consequence of histone exchange |
|
| Relations |
| BioProject |
PRJNA141739 |
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