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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 03, 2011 |
| Title |
Genetic and pharmacologic approach to identify genes regulated by mTORC1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a critical regulator of cell growth by integrating multiple signals (nutrients, growth factors, energy and stress) and is frequently deregulated in many types of cancer. We used a robust experimental paradigm involving the combination of two interventions, one genetic and one pharmacologic to identify genes regulated transcriptionally by mTORC1. In Tsc2+/+, but not Tsc2-/- immortalized mouse embryo fibroblasts (MEFs), serum deprivation downregulates mTORC1 activity. In Tsc2-/- cells, abnormal mTORC1 activity can be downregulated by treatment with rapamycin (sirolimus). By contrast, rapamycin has little effect on mTORC1 in Tsc2+/+ cells in which mTORC1 is already inhibited by low serum. Thus, under serum deprived conditions, mTORC1 activity is low in Tsc2+/+ cells (untreated or rapamycin treated), high in Tsc2-/- cells, but lowered by rapamycin; a pattern referred to as a “low/low/high/low” or “LLHL”, which allowed the identification of genes regulated by mTORC1 by performing the appropriate comparisons
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| Overall design |
Mouse embryo fibroblasts (MEFs) wild type or deficient in Tsc2 were treated with 25 nM rapamycin or vehicle (methanol) for 24 h under low serum conditions (0.1% FBS)
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| Contributor(s) |
Peña-Llopis S, Brugarolas J |
| Citation(s) |
21804531 |
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| Submission date |
Mar 16, 2011 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Samuel Peña-Llopis |
| E-mail(s) |
[email protected], [email protected]
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| Phone |
(01) 214-648-4099
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| Fax |
(01) 214-648-1960
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| Organization name |
UT Southwestern Medical Center
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| Department |
Internal Medicine - Oncology Division
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| Lab |
Brugarolas Lab
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| Street address |
6000 Harry Hines Blvd., NB5.102
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9133 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| GSM692435 |
wt MEFs, rapamycin, rep2 |
| GSM692436 |
Tsc2-/- MEFs, vehicle, rep1 |
| GSM692437 |
Tsc2-/- MEFs, vehicle, rep2 |
| GSM692438 |
Tsc2-/- MEFs, rapamycin, rep1 |
| GSM692439 |
Tsc2-/- MEFs, rapamycin, rep2 |
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| Relations |
| BioProject |
PRJNA137809 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27982_RAW.tar |
27.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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