|
| Status |
Public on May 24, 2005 |
| Title |
Brassinosteroid mutant (bzr1-1D and det2) analysis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth
and development of plants. BR signaling through cell-surface receptor
kinases and intracellular components leads to dephosphorylation and
accumulation of the nuclear protein BZR1. How BR signaling regulates gene
expression, however, remains unknown. Here we show that BZR1 is a
transcriptional repressor that has a previously unknown DNA binding domain
and binds directly to the promoters of feedback-regulated BR biosynthetic
genes. To identify additional BZR1-regulated genes and to understand the
BR-regulated transcriptional pathways, we examined the effects of bzr1-1D
and det2 mutations on the expression of BR-regulated genes by using the
Arabidopsis full-genome oligo microarray (Affymetrix). Microarray analyses
identified additional potential targets of BZR1 and illustrated, together
with physiological studies, that BZR1 coordinates BR homeostasis and
signaling by playing dual roles in regulating BR biosynthesis and downstream
growth responses.
Keywords: other
|
| |
|
| |
|
| Contributor(s) |
Gendron JM, Wang Z |
| Citation(s) |
15681342 |
| |
| Submission date |
May 23, 2005 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Joshua Martin Gendron |
| E-mail(s) |
[email protected]
|
| Phone |
(650)325-1521
|
| Organization name |
Carnegie Institution of Washington/Stanford University
|
| Department |
Plant Biology
|
| Lab |
Wang
|
| Street address |
260 Panama Rd.
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA92337 |
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