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| Status |
Public on Jan 25, 2012 |
| Title |
Cell cycle expression profiles in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Periodic expression of cell cycle genes highlights the importance of precise temporal control of transcription in regulating cell cycle events. We used HeLa cells enriched for different phases of the cell cycle to identify genes that are expressed in a periodic fashion in specific cell cycle phases. These data were generated for comparison with ChIP-Sequencing data obtained for two subunits of the B-Myb-MuvB complex, B-Myb and LIN9, in HeLa cells.
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| Overall design |
HeLa cells were synchronized at the beginning of S phase by double thymidine block and then released into the cell cycle by washing out the thymidine resulting in tight synchrony at S, G2 and M phases of the cell cycle. Most cells entered mitosis at 8 hours after release as determined by visual inspection. Three independent replicas of double thymidine block and release experiments were performed. RNA was isolated at specific times after release from the thymidine block (0, 2, 4, 6, 8 and 12 hours) and used for generating expression profiles.
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| Contributor(s) |
Sadasivam S, DeCaprio JA |
| Citation(s) |
22391450 |
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| Submission date |
Jan 27, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Subhashini Sadasivam |
| E-mail(s) |
[email protected]
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| Organization name |
Dana Farber Cancer Insitute
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| Department |
Medical Oncology
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| Lab |
Laboratory of James DeCaprio, Mayer 444
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| Street address |
450 Brookline Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM662895 |
HeLa cells blocked, biological rep1 |
| GSM662896 |
HeLa cells blocked, biological rep2 |
| GSM662897 |
HeLa cells blocked, biological rep3 |
| GSM662898 |
HeLa cells 2h released, biological rep1 |
| GSM662899 |
HeLa cells 2h released, biological rep2 |
| GSM662900 |
HeLa cells 2h released, biological rep3 |
| GSM662901 |
HeLa cells 4h released, biological rep1 |
| GSM662902 |
HeLa cells 4h released, biological rep2 |
| GSM662903 |
HeLa cells 4h released, biological rep3 |
| GSM662904 |
HeLa cells 6h released, biological rep1 |
| GSM662905 |
HeLa cells 6h released, biological rep2 |
| GSM662906 |
HeLa cells 6h released, biological rep3 |
| GSM662907 |
HeLa cells 8h released, biological rep1 |
| GSM662908 |
HeLa cells 8h released, biological rep2 |
| GSM662909 |
HeLa cells 8h released, biological rep3 |
| GSM662910 |
HeLa cells 12h released, biological rep1 |
| GSM662911 |
HeLa cells 12h released, biological rep2 |
| GSM662912 |
HeLa cells 12h released, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE27031 |
The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression |
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| Relations |
| BioProject |
PRJNA142161 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26922_RAW.tar |
88.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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