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| Status |
Public on Jun 24, 2011 |
| Title |
SMAD3 targets in A549 lung epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
TGFβ activates a signal transduction cascade that results in the transcription of genes, primarily through the DNA-binding transcription factor SMAD3. The objective of this study is to identify SMAD3 binding targets and the molecular pathways affected by the TGFβ1/SMAD3 signal transduction on a genome-wide scale.
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| Overall design |
Cultured A549 cells at 30-50% confluence were treated with 10 uM SIS3 in dimethyl sulfoxide (DMSO), or DMSO (vehicle-only) 30 min prior to TGFβ1 treatment. Cells were treated with 2 ng/mL recombinant TGFβ1 for 0, 2, 12, and 24 h.
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| Contributor(s) |
Zhang Y, Handley D, Pandit KV, Kaminski N |
| Citation(s) |
21625455 |
| |
| Submission date |
Jan 25, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
[email protected]
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (19)
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| GSM661204 |
A549 cells_0h control_biological rep1 |
| GSM661205 |
A549 cells_0h control_biological rep2 |
| GSM661206 |
A549 cells_0h control_biological rep3 |
| GSM661207 |
A549 cells_2h control_biological rep1 |
| GSM661208 |
A549 cells_2h control_biological rep2 |
| GSM661209 |
A549 cells_2h control_biological rep3 |
| GSM661210 |
A549 cells_12h control_biological rep1 |
| GSM661211 |
A549 cells_12h control_biological rep2 |
| GSM661212 |
A549 cells_24h control_biological rep1 |
| GSM661213 |
A549 cells_24h control_biological rep2 |
| GSM661214 |
A549 cells_24h control_biological rep3 |
| GSM661215 |
A549 cells_0h SIS3_biological rep1 |
| GSM661216 |
A549 cells_0h SIS3_biological rep2 |
| GSM661217 |
A549 cells_0h SIS3_biological rep3 |
| GSM661218 |
A549 cells_12h SIS3_biological rep1 |
| GSM661219 |
A549 cells_12h SIS3_biological rep2 |
| GSM661220 |
A549 cells_24h SIS3_biological rep1 |
| GSM661221 |
A549 cells_24h SIS3_biological rep2 |
| GSM661222 |
A549 cells_24h SIS3_biological rep3 |
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| Relations |
| BioProject |
PRJNA135983 |
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