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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 10, 2011 |
| Title |
Effect of Glis1, Dmrtb1, and Pitx2 on mouse iPS cell generation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Induced pluripotent stem cells (iPSC) are generated from somatic cells by the transgene expression of three transcription factors Oct3/4, Sox2, and Klf4 (OSK), albeit at a low efficiency. The protooncogene c-Myc enhances the efficiency of iPSC generation by OSK, but it also increases the tumorigenicity of the resulting iPSC.
In the current study, we found the Gli-like transcription factor Glis1, when expressed together with OSK, to markedly enhance the generation of iPSC from both mouse and human fibroblasts. Mouse iPSC generated by OSK and Glis1 can form germline-competent chimeras. Glis1 is enriched in unfertilized oocytes and one cell-stage embryos. DNA microarray analyses revealed that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, mesenchymal-epithelial transition (MET), and Esrrb. These results therefore demonstrated that oocyte transcription factor Glis1 effectively promote direct reprogramming during iPSC generation.
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| Overall design |
Mouse embryonic fibroblasts were transduced with OSKM, OSM+Glis1, OSM+Dmrtb1, and OSM+Pitx2 and were used for microarray analyses.
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| Contributor(s) |
Maekawa M, Nakamura T, Nrita M |
| Citation(s) |
21654807 |
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| Submission date |
Jan 04, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
[email protected]
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| Phone |
81-75-366-7041
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| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
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| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA142263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26430_RAW.tar |
39.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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