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| Status |
Public on Nov 22, 2024 |
| Title |
Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and astrocytic function [bulkRNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have developed a panel of isogenic iPSC lines carrying MAPT splice-site mutations S305S, S305I or S305N, derived from four different donors. All three mutations significantly increased the proportion of 4R tau expression in iPSC-neurons and astrocytes, with up to 80% 4R transcripts in S305N neurons from as early as 4 weeks of differentiation.
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| Overall design |
We performed bulk RNA-sequencing on isogenic sets of MAPT S305 mutation iPSC-neurons at 4, 6 and 8 weeks of differentiation, as well as iPSC-astrocytes
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| Contributor(s) |
Bowles KR, Goate AM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 28, 2024 |
| Last update date |
Nov 22, 2024 |
| Contact name |
Kathryn R Bowles |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Street address |
49 Little France Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4SB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (83)
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| This SubSeries is part of SuperSeries: |
| GSE279322 |
Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and astrocytic function |
|
| Relations |
| BioProject |
PRJNA1081921 |
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